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February 15, 2020

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Construction of a library of inducible recombinases

May 18, 2019

The Wong lab and Dr. Jake Beal have constructed a library >20 orthogonal inducible split recombinases that can be activated by small molecules, light, and temperature in mammalian cells and mice.  Using the library of inducible recombinase, they create a genetic switchboard that can independently regulate the expression of 3 different cytokines in the same cell, a tripartite inducible Flp, and a 4-Input AND gate. 

This library has been deposited on Addgene.

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© 2020 Living Computing Project.

Sponsored by National Science Foundation’s Expeditions in Computing Program

(Awards #1522074 / 1521925 / 1521759).