My working title for the paper that will come from this year’s iGEM interlab study is “Relative Fluorescent Units Considered Harmful.” It’s a bit of a playful title, invoking a computer science tradition started by the notorious Edsger Dijkstra. I think, however, that this statement is deserved and also that we can now back it up with some hard experimental evidence.
Most of the data is in—I’m just waiting for a few more teams with extensions—and it looks like we’ve got amazing results. The big news is, in a more positive reformulation of my title, that calibrating fluorescence measurements works, and that it makes a big enough difference to be worth it. Let me present the key conclusions that I believe we can now support in the form of responses to the most common arguments that I hear against calibrating fluorescent measurements.
Read Beal's full blog post here.